منابع مشابه
Interaction of calmodulin with adrenal chromaffin granule membranes.
We have reported that calmodulin bound specifically to catecholamine-containing secretory granules of adrenal medullary chromaftin cells [I]. Binding was dependent upon pM levels of Ca2+ and saturated at a low level (3.3. pmol calmodulin/mg granule membrane protein). Additionally, we observed that the phosphorylation of membrane polypeptides of M, 59 000, 58 000 and 53 000 was stimulated by cal...
متن کاملTensile strength of the chromaffin granule membrane.
Catecholamine release from chromaffin granules, suspended in sucrose solutions of various osmotic strengths, was determined at different temperatures between 2 degrees and 44 degrees C. Dynamic measurements showed that steady state is achieved within 15 min of incubation at all temperatures. The effect of temperature on the release was established in terms of the median granular fragility (MGF)...
متن کاملPlasma membrane and chromaffin granule characteristics in digitonin-treated chromaffin cells.
Digitonin permeabilizes the plasma membranes of bovine chromaffin cells to Ca2+, ATP, and proteins and allows micromolar Ca2+ in the medium to stimulate directly catecholamine secretion. In the present study the effects of digitonin (20 microM) on the plasma membrane and on intracellular chromaffin granules were further characterized. Cells with surface membrane labeled with [3H]galactosyl moie...
متن کاملHydroxytryptamine transport by the bovine chromaffin-granule membrane.
5-Hydroxytryptamine is accumulated by resealed chromaffin-granule 'ghosts' if a pH gradient (acid inside) is imposed across their membranes by preincubating them at low pH. This uptake, like that driven by MgATP, is reserpine- and uncoupler-sensitive. This strongly suggests that catecholamines are taken up by intact granules in response to a pH gradient. In line with this, it is shown that 5-hy...
متن کاملPassive ion permeability of the chromaffin-granule membrane.
'Ghosts' of bovine chromaffin granules, in which the complex mixture of proteins and solutes normally found in the granule matrix is replaced by buffered sucrose are osmotically sensitive. They shrink when the osmotic pressure of the suspension medium is increased, and swell if solute entry is facilitated by the addition of ionophores. Swelling in the presence of ionophores has been used to inv...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1985
ISSN: 0014-5793
DOI: 10.1016/0014-5793(85)80258-1